EST-SSR markers from five sequenced cDNA libraries of common bean (Phaseolus vulgaris L.) comparing three bioinformatic algorithms.

@article{Blair2013ESTSSRMF,
  title={EST-SSR markers from five sequenced cDNA libraries of common bean (Phaseolus vulgaris L.) comparing three bioinformatic algorithms.},
  author={Matthew W Blair and N. Cabeza Hurtado},
  journal={Molecular ecology resources},
  year={2013},
  volume={13 4},
  pages={
          688-95
        }
}
Expressed sequence tags (ESTs) are a rich source of SSR sequences, but the proportion of long Class I microsatellites with many repeats vs. short Class II microsatellites with few repeats is an important factor to consider. Class I microsatellites, with more than 20 bp of repeats, tend to make better markers with higher polymorphism. The goal of this study was to determine the frequency of Class I and Class II microsatellites in a collection of over 21,000 ESTs from a single study of five… CONTINUE READING

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