ESR1 Amplification in Breast Cancer by Optimized RNase FISH: Frequent but Low-Level and Heterogeneous

@inproceedings{Moelans2013ESR1AI,
  title={ESR1 Amplification in Breast Cancer by Optimized RNase FISH: Frequent but Low-Level and Heterogeneous},
  author={Cathy B Moelans and Frederik Holst and Olaf Jos{\'e}-Carlos Hellwinkel and Ronald Simon and Paul J van Diest},
  booktitle={PloS one},
  year={2013}
}
Prevalence of ESR1 amplification in breast cancer is highly disputed and discrepancies have been related to different technical protocols and different scoring approaches. In addition, pre-mRNA artifacts have been proposed to influence outcome of ESR1 FISH analysis. We analyzed ESR1 gene copy number status combining an improved RNase FISH protocol with multiplex ligation-dependent probe amplification (MLPA) after laser microdissection. FISH showed a high prevalence of ESR1 gains and… CONTINUE READING

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