ESI-MS assay of M. tuberculosis cell wall antigen 85 enzymes permits substrate profiling and design of a mechanism-based inhibitor.

Abstract

Mycobacterium tuberculosis Antigen 85 enzymes are vital to the integrity of the highly impermeable cell envelope and are potential therapeutic targets. Kinetic analysis using a label-free assay revealed both mechanistic details and a substrate profile that allowed the design and construction of a selective in vitro mechanism-based inhibitor.

DOI: 10.1021/ja204249p

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Cite this paper

@article{Barry2011ESIMSAO, title={ESI-MS assay of M. tuberculosis cell wall antigen 85 enzymes permits substrate profiling and design of a mechanism-based inhibitor.}, author={Conor St J Barry and Keriann M. Backus and Clifton Earl Barry and Benjamin G Davis}, journal={Journal of the American Chemical Society}, year={2011}, volume={133 34}, pages={13232-5} }