ESI-FTICR mass spectrometry employing data-dependent external ion selection and accumulation.


Data-dependent external m/z selection and accumulation of ions is demonstrated in use with ESI-FTICR instrumentation, with two different methods for ion selection being explored. One method uses RF/DC quadrupole filtering and is described in use with an 11.5 tesla (T) FTICR instrument, while the second method employs RF-only resonance dipolar excitation selection and is described in use with a 3.5 T FTICR instrument. In both methods ions are data-dependently selected on the fly in a linear quadrupole ion guide, then accumulated in a second linear RF-only quadrupole trap that immediately follows. A major benefit of ion preselection prior to external accumulation is the enhancement of ion populations for low-level species. This development is expected to expand the dynamic range and sensitivity of FTICR for applications including analysis of complex polypeptide mixtures (e.g., proteomics).

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@article{Harkewicz2002ESIFTICRMS, title={ESI-FTICR mass spectrometry employing data-dependent external ion selection and accumulation.}, author={Richard Harkewicz and Mikhail E. Belov and Gordon A. Anderson and Ljiljana Pasa-Tolic and Christophe D Masselon and David C. Prior and Harold R. Udseth and Richard D. Smith}, journal={Journal of the American Society for Mass Spectrometry}, year={2002}, volume={13 2}, pages={144-54} }