EPR Studies of V-ATPase with Spin-Labeled Inhibitors DCC and Archazolid: Interaction Dynamics with Proton Translocating Subunit c.

@article{Glz2016EPRSO,
  title={EPR Studies of V-ATPase with Spin-Labeled Inhibitors DCC and Archazolid: Interaction Dynamics with Proton Translocating Subunit c.},
  author={Jan Philipp G{\"o}lz and Svenja Bockelmann and Kerstin Mayer and Heinz-Juergen Steinhoff and Helmut Wieczorek and Markus Huss and Johann P. Klare and Dirk Menche},
  journal={ChemMedChem},
  year={2016},
  volume={11 4},
  pages={420-8}
}
Vacuolar-type H(+) -ATPases (V-ATPases) have gained recent attention as highly promising anticancer drug targets, and therefore detailed structural analyses and studies of inhibitor interactions are very important research objectives. Spin labeling of the V-ATPase holoenzyme from the tobacco hornworm Manduca sexta and V-ATPase in isolated yeast (Saccharomyces cerevisiae) vacuoles was accomplished by two novel methods involving the covalent binding of a (2,2,6,6-tetramethylpiperidin-1-yl)oxyl… CONTINUE READING