EMS and UV mutagenesis in yeast.

  title={EMS and UV mutagenesis in yeast.},
  author={Fred Winston},
  journal={Current protocols in molecular biology},
  volume={Chapter 13},
  pages={Unit 13.3B}
Many fundamental biological processes have been elucidated by the isolation and analysis of mutants that are defective in such processes. Therefore, the methods to generate mutants are of great importance in model organisms. This unit describes two protocols for mutagenesis of yeast-using ethyl methanesulfate (EMS) and ultraviolet (UV) light. Each of these… CONTINUE READING