EMD 52692 (bimakalim), a new potassium channel opener, attenuates luminol-enhanced chemiluminescence and superoxide anion radical formation by zymosan-activated polymorphonuclear leukocytes.

  title={EMD 52692 (bimakalim), a new potassium channel opener, attenuates luminol-enhanced chemiluminescence and superoxide anion radical formation by zymosan-activated polymorphonuclear leukocytes.},
  author={Galen M. Pieper and Garrett John Gross},
  volume={23 3},

The Small G-protein Rac Mediates Depolarization-induced Superoxide Formation in Human Endothelial Cells*

It is concluded that depolarization is an important stimulus of endothelial superoxide production, which involves a tyrosine phosphorylation-dependent translocation of the small G-protein Rac.

Adenosine inhibition of neutrophil damage during reperfusion does not involve KATP-channel activation.

It is concluded that adenosine A2 activation before reperfusion partially reduces infarct size by inhibiting neutrophil activity and that this effect does not involve KATP-channel stimulation.

Isoflurane Inhibits Neutrophil-Endothelium Interactions in the Coronary Circulation: Lack of a Role for Adenosine Triphosphate-Sensitive Potassium Channels

Isoflurane had an inhibitory action on neutrophil-endothelium interactions and neutrophils-mediated coronary endothelial dysfunction—effects that may be involved in its cardioprotective action in vivo.

Depolarization-associated iron release with abrupt reduction in pulmonary endothelial shear stress in situ.

ROS generation and iron release with ischemia are independent although both are subsequent to endothelial cell membrane depolarization, indicating that ROS generation is not required for ischemic-mediated iron release.

Influence of ion channel modulation on in vitro interferon-gamma induced MHC class I and II expression on macrophages.

The in vitro effect of K+ channel blockers quinidine and verapamil, anion channel blocker SITS and K+ channel openers diazoxide, pinacidil, and BRL 38227 on interferon-gamma (IFN-gamma) induced MHC

Role for NADPH/NADH Oxidase in the Modulation of Vascular Tone a

Under all conditions of an activated circulating and/or local renin‐angiotensin system endothelial dysfunction secondary to increased vascular O2−• production is expected, and dynamic interactions may likely have important implications for tissue perfusion and the process of atherosclerosis.

Bimakalim, an ATP-sensitive potassium channel opener, mimics the effects of ischemic preconditioning to reduce infarct size, adenosine release, and neutrophil function in dogs.

Results indicate that myocardial preconditioning in the canine heart produced by a short period of ischemia or a KATP channel opener is not mediated by an increase inAdenosine release, as measured by coronary venous adenosine concentrations, during 60 minutes of occlusion or the initial 30 minutes of reperfusion.

Effect of adenosine triphosphate-sensitive potassium channel openers on lung preservation.

It is suggested that KATP openers would maintain the mitochondrial respiratory function during lung preservation, prevent lipid peroxidation after reperfusion, and attenuate ischemia-reperfusion injury.



Independent stimulation of membrane potential changes and the oxidative metabolic burst in polymorphonuclear leukocytes.

According to this data, membrane depolarization may antecede oxidative responses in PMNL, but appears to depend upon the method of cell preparation and the nature of the stimulus.

Linoleic acid-deficient rat neutrophils show decreased bactericidal capacity, superoxide formation and membrane depolarization.

It is shown that neutrophils from EFAD rats exhibited impairments of membrane depolarization and superoxide anion formation upon stimulation with a formylpeptide, FMLP, or when challenged with living Staphylococcus aureus (but not when activated with phorbol myristate acetate).

Platelets as Scavengers of Neutrophil-Derived Oxidants: A Possible Defence Mechanism at Sites of Vascular Injury

It is suggested that PLTs have the capacity of limiting the oxidant production by PMNs, and may represent a natural device for the protection of vascular structures from PMN-mediated oxidative stresses.

Relationship between membrane potential changes and superoxide-releasing capacity in resident and activated mouse peritoneal macrophages.

The findings indicate that membrane potential changes are closely associated with O2- -releasing capacity in macrophages, and that the systems that mediate membranes potential changes and production of O2 -release develop or decline concomitantly during activation or deactivation of the cells.

Voltage-dependent and Ca2(+)-activated ion channels in human neutrophils.

The Ca2(+)-activated K+ and Cl- channels may play a role in cell volume homeostasis and/or cellular activation, and are investigated for their role in membrane voltage and transmembrane ion fluxes in human neutrophils.

PMN heterogeneity: long-term stability of fluorescent membrane potential responses to the chemoattractant N-formyl-methionyl-leucyl-phenylalanine in healthy adults and correlation with respiratory burst activity.

The data indicate that PMNs from healthy adults demonstrate a heterogeneous membrane potential response to the chemotaxin FMLP that correlates with the cells' oxidative responsiveness and that intersubject differences can be detected.

Changes in membrane potential of human granulocytes antecede the metabolic responses to surface stimulation.

  • H. KorchakG. Weissmann
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1978
The data suggest that DeltaPsi is involved in primary triggering of phagocytic cells and that metabolic stimulation is a secondary consequence of ligand-receptor interactions.

Prostaglandin E1 and prostaglandin I2 modulation of superoxide production by human neutrophils.