ELISA test, a new method to detect and quantify isoproturon in soil.

@article{Ligeois1992ELISATA,
  title={ELISA test, a new method to detect and quantify isoproturon in soil.},
  author={E Li{\'e}geois and Y Dehon and B de Brabant and Paul Perry and Daniel Portetelle and Alfred Copin},
  journal={The Science of the total environment},
  year={1992},
  volume={123-124},
  pages={17-28}
}
An indirect competitive Enzyme-Linked ImmunoSorbent Assay (ELISA) has been developed to quantify isoproturon (a commonly used phenylurea herbicide) in soil samples. To produce the specific antibodies needed for detection of isoproturon, an isopropylanilin analogue was covalently linked to a proteic carrier and the resulting conjugate used as immunogen. Hybridomas were obtained from spleen cells of mice immunized with this conjugate. One specific monoclonal antibody (MAb) was selected and used… CONTINUE READING