ELISA for the detection of venoms from four medically important snakes of India.

@article{Selvanayagam1999ELISAFT,
  title={ELISA for the detection of venoms from four medically important snakes of India.},
  author={Zachariah E Selvanayagam and S G Gnanavendhan and Koluman A Ganesh and Deepa Rajagopal and Putcha V. Lakshmana Rao},
  journal={Toxicon : official journal of the International Society on Toxinology},
  year={1999},
  volume={37 5},
  pages={757-70}
}
A double antibody sandwich enzyme linked immunosorbent assay (ELISA) was developed to detect Echis carinatus venom in various organs (brain, heart, lungs, liver, spleen and kidneys) as well as tissue at the site of injection of mice, at various time intervals (1, 6, 12, 18, 24 h and 12 h intervals up to 72 h) after death. The assay could detect E. carinatus venom levels up to 2.5 ng/ml of tissue homogenate and the venom was detected up to 72 h after death. A highly sensitive and species… CONTINUE READING
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