ELISA and HPLC determination of the occurrence of aflatoxin M(1) in raw cow's milk.

@article{Velasco2003ELISAAH,
  title={ELISA and HPLC determination of the occurrence of aflatoxin M(1) in raw cow's milk.},
  author={M L Rodr{\'i}guez Velasco and M M Calonge Delso and D Ord{\'o}{\~n}ez Escudero},
  journal={Food additives and contaminants},
  year={2003},
  volume={20 3},
  pages={276-80}
}
Raw cow's milk collected from dairy farms in the province of Leon, Spain, was examined for aflatoxin M(1) (AFM(1)). The samples were analysed with a commercial competitive enzyme-linked immunosorbent assay (ELISA) kit and high-performance liquid chromatography (HPLC). The concentrations of AFM(1) in the milk extracts were initially estimated by ELISA, with recovery rates of 74.6-109% for artificially contaminated milk at levels of 10-80 ng x l(1). Samples found to contain more than 10 ng x l(1… CONTINUE READING

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