Corpus ID: 78723238

ELISA- A Mini Review

  title={ELISA- A Mini Review},
  author={A. Baid},
The ELISA (Enzyme Linked Immunosorbent Assay) is a strategy versatile framework for quantitation of antigens and antibodies. The segregation of antibodies to understand substance structures helped ELISA unbounded uses of analytic estimation. 
Capture-Layer Lateral Flow Immunoassay: A New Platform Validated in the Detection and Quantification of Dengue NS1
This method shows another form of LFIA that has the potential to be quantitative (at least semiquantitative), albeit not solving the reader cost; however, unlike the regular LFIA, this method does not use nanobeads but instead enzymes, allowing, in theory, greater sensitivity, while retaining the one-step procedure. Expand
Microfluidic devices for sample preparation and rapid detection of foodborne pathogens.
Recent advances in current state-of-the-art of sample preparation and concentration from food matrices with focus on bacterial capturing methods and sensing technologies, along with their advantages and limitations when integrated into microfluidic devices for online rapid detection of pathogens in foods and food production line are reviewed. Expand


Competitive Enzyme Linked Immuno-Sorbent Assay (c-ELISA) Based Sero-Prevalence of Bluetongue Virus (BTV) on Small Ruminants in SelectedAreas of Wolyita, Southern Ethiopia
This is the first report indicating the presence of bluetongue virus infection in the area, andSerum samples collected randomly from the accessed small ruminates screened for detection of BTV-specific immunoglobulin G (IgG) antibodies using a competitive enzyme-linked immunosorbent assay (c-ELISA). Expand
A rapid ELISA based method to determine peptidyl-arginine deiminase activity in biological samples
This new method is based on the binding of an antibody specifically recognising a citrulline-containing epitope in a defined peptide and can be applied to monitor PAD activity in many types of biological samples, such as bacterial lysates, mammalian cell extracts and tissue extracts. Expand
Uncertainty of Measurement for ELISA in a Serological Testing Laboratory
Background: Analytical results estimating Uncertainty of Measurement (MU) signifies the confidence level of the concerned assay. Enzyme-linked immunosorbent assay (ELISA) is an extensively usedExpand
A Rapid ELISA Method to Improve the Automated Test Throughput
In ELISA, ligand is commonly adsorbed to the plastic surface through non-covalent bonds between the hydrophobic regions of the ligand and the plastic surface. Thus, all the reactions occur in aExpand
A New Sandwich ELISA Test Simultaneously Detecting E7 Proteins of HPV-16,18 and 45 in Cervical Smears
A new Enzyme-Linked Immunosorbent Assay (ELISA)-based detection system to monitor expression of the E7 proteins of the high-risk HPV (hrHPV) types HPV-16, HPV-18, and HPV-45 in cervical smears is generated. Expand
Environmental and occupational respiratory diseases – 1034. A novel ELISA method for the detection of dust mite allergens using IgY technology
This study standardizes a in house method of indirect ELISA assay for detection of mite allergens using mite IgY antibodies for atopic patients sensitized to house dust mites. Expand
The Development and Application of ELISA for 3-Indole-Butyric Acid
Indole-3-butyric acid (IBA) is a plant growth hormone used to improve the formation of roots during the culture of cuttings in the greenhouse. According to new EC regulations, IBA levels shouldExpand
Comparison of multiplex, ELISA and immunofluorescence for detecting autoantibodies in JIA patients
Results from IFA testing with a multiplex antinuclear antibody assay on a panel of patient serum samples previously evaluated by 3 commercial ANA ELISAs are compared. Expand
Evaluation of Two Different Screening ELISA Assays for Synthetic Cathinones (Mephedrone/Methcathinone and MDPV) with LC-MS Method in Intoxicated Patients
  • 2016
Context: Synthetic cathinones are a new trend in the recreational drug market and the paucity of human toxicological data combined with their widespread abuse generated great concern in theExpand
Adverse reaction to food screening test: new multiparametric ELISA kit for patients with gastrointestinal symptomatology linked to ingesting food
The adverse reaction to food screening test appears to be a kit providing an innovative approach for single patients, with interesting clinical applications and considerable savings from an economic point of view. Expand