ELECTRON SHADOW MICROGRAPHY OF THE TOBACCO MOSAIC VIRUS PROTEIN

@article{Williams1945ELECTRONSM,
  title={ELECTRON SHADOW MICROGRAPHY OF THE TOBACCO MOSAIC VIRUS PROTEIN},
  author={R. C. Williams and Ralph Walter Graystone Wyckoff},
  journal={Science},
  year={1945},
  volume={101},
  pages={594 - 596}
}
Two improvements are described in the use of shadow electron micrography for the observation of particles of macromolecular dimensions. One involves the substitution of gold for chromium as shadowing metal. The thinner gold film that can be employed gives a truer representation of the shape of particles so small that shape and size are significantly altered by the thickness of the shadowing metal deposited on them. The other consists in metal-shadowing small particles deposited on a very smooth… 

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References

SHOWING 1-2 OF 2 REFERENCES

Electron Shadow-Micrography of Virus Particles.*

A new procedure is described for the electron microscopy of small objects that involves the oblique evaporation of a thin film of metal over the preparation before micrography, which produces new information about the heights and shapes of objects seen in the preparation.