EHMT1 protein binds to nuclear factor-κB p50 and represses gene expression.


Transcriptional homeostasis relies on the balance between positive and negative regulation of gene transcription. Methylation of histone H3 lysine 9 (H3K9) is commonly correlated with gene repression. Here, we report that a euchromatic H3K9 methyltransferase, EHMT1, functions as a negative regulator in both the NF-κB- and type I interferon-mediated gene induction pathways. EHMT1 catalyzes H3K9 methylation at promoters of NF-κB target genes. Moreover, EHMT1 interacts with p50, and, surprisingly, p50 appears to repress the expression of type I interferon genes and genes activated by type I interferons by recruiting EHMT1 to catalyze H3K9 methylation at their promoter regions. Silencing the expression of EHMT1 by RNA interference enhances expression of a subset NF-κB-regulated genes, augments interferon production, and augments antiviral immunity.

DOI: 10.1074/jbc.M112.365601

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@article{Ea2012EHMT1PB, title={EHMT1 protein binds to nuclear factor-κB p50 and represses gene expression.}, author={Chee-Kwee Ea and Shengli Hao and Kok Siong Yeo and David Baltimore}, journal={The Journal of biological chemistry}, year={2012}, volume={287 37}, pages={31207-17} }