EGF-induced EGF-receptor and MAP kinase phosphorylation in goat cumulus cells during in vitro maturation.

@article{Gall2005EGFinducedEA,
  title={EGF-induced EGF-receptor and MAP kinase phosphorylation in goat cumulus cells during in vitro maturation.},
  author={Laurence Gall and Claire Boulesteix and Sylvie Ruffini and Guy Germain},
  journal={Molecular reproduction and development},
  year={2005},
  volume={71 4},
  pages={
          489-94
        }
}
EGF has been shown to influence meiotic maturation and development competence of oocyte in various mammalian species. We previously reported, in goat, that the EGF receptor (EGF-R) was present both on cumulus cells and oocytes. Here, EGF-induced signaling was investigated during the in vitro maturation process in goat cumulus-oocyte complexes (COCs). Cumulus cells and oocytes were subjected to Western immunoblotting analysis using anti-MAP kinase, anti-phosphotyrosine, anti-phospho MAP kinase… CONTINUE READING

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