Corpus ID: 36173048

EFFICIENT IN VITRO PLANT REGENERATION OF PINELLIA TERNATA (THUNB) BREIT

@article{Xu2005EFFICIENTIV,
  title={EFFICIENT IN VITRO PLANT REGENERATION OF PINELLIA TERNATA (THUNB) BREIT},
  author={Tiefeng Xu and Lei Zhang and Xiao-fen Sun and Kexuan Tang},
  journal={Acta Biologica Cracoviensia Series Botanica},
  year={2005},
  volume={47}
}
An efficient plant regeneration system for Pinellia ternata (Thunb) Breit, an important wild Chinese herb, was established. The investigated factors influencing callus induction and plant regeneration included sterilizing agents, explant type, culture medium, and combinations of plant growth regulators in the medium. Ten min treatment of explants with 0.1% HgCl2 was more suitable for sterilization of tuber explants than NaClO and H2O2. Tubers as explants were superior to leaves and petioles… Expand

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References

SHOWING 1-10 OF 29 REFERENCES
Plant regeneration via organogenesis in marigold
TLDR
Thirteen days was the best time of exposure of the explant to the regeneration medium for shoot induction and the highest values for regeneration were obtained with BA (13.3 μM) and IAA (17.1 μM). Expand
Stimulatory effect of water stress on plant regeneration in aromatic Indica rice varieties
TLDR
Frequency of regeneration of fertile plants from cell suspension-derived calli was significantly increased using water stress treatments in two commercially cultivated Indian aromatic rice varieties and a similar stimulatory effect of water stress on plant regeneration was observed in another Indica rice variety, IR43, and a Japonica rice varieties, Taipei 309. Expand
Protoplast culture and plant regeneration ofPinellia ternata
TLDR
K8P and modified MS media were used to culture protoplasts in: a) liquid, b) liquid-solid double layer, or c) agarose embedded protoplast culture, and the former two were conducive to colony formation fromprotoplast-derived cells. Expand
Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources
The experiments have revealed that (NH4)2SO4 combined with KNO3 at low concentration is of advantage to the formation, growth and differentiation of pollen callus in rice, whereas the highExpand
Simple dehydration treatment promotes plantlet regeneration of rice (Oryza sativa L.) callus
SummaryTo increase plantlet regeneration frequency, rice callus was dehydrated in a Petri dish with a single layer of filter paper prior to transfer to the regeneration medium. With a 24 hExpand
Factors influencing plant regeneration from protoplasts isolated from long-term cell suspension culture of recalcitrant Indica rice cultivar IR36
TLDR
The utilization of a two-step regeneration procedure led to regeneration of fertile plants from protoplasts isolated from 2-yr-old cell suspensions of IR36, one of the most important but recalcitrant rice cultivars. Expand
Plant tissue culture media
TLDR
The development of the various types of tissue culture has been based on empirical approaches, and some of the observations recorded in the literature may not be typical for plant cells. Expand
[Progress on research of rapid propagation system of Pinellia ternata].
  • Z. Mao, Z. Peng
  • Biology, Medicine
  • Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
  • 2003
TLDR
Traditional rapid propagation system of Pinellia ternata may be improved from the following aspects: the best cooperation of hormones, the most active ex-plant, one time culture and suitable transplant. Expand
Herbicide Use on Roundup Ready Crops
TLDR
Although the total amount of herbicides used with soybeans has changed little with the introduction of Roundup Ready varieties, the data show a substantial reduction in the number of applications made to soybean acreage, and this demonstrates growers using fewer active ingredients and making fewer trips over the field, which translates into ease of management. Expand
Quantitative analysis of anti-emetic principle in the tubers of Pinellia ternata by enzyme immunoassay.
TLDR
6KDP is a marker for quantitative analysis of the tubers of P. ternata and demonstrates a low cross-reaction with the crude globulin fraction of Colocasia antiquorum, a member of the same family as P. Ternata. Expand
...
1
2
3
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