• Corpus ID: 87252348

EFFECT OF VIBRIO ANGUILLARUM ON ACTIVITY AND GENE EXPRESSION OF GLUTATHIONE S-TRANSFERASES IN CYCLINA SINENSIS

@inproceedings{Kai2012EFFECTOV,
  title={EFFECT OF VIBRIO ANGUILLARUM ON ACTIVITY AND GENE EXPRESSION OF GLUTATHIONE S-TRANSFERASES IN CYCLINA SINENSIS},
  author={Luo Kai},
  year={2012}
}

• Luo Kai
• Published 2012
• Biology

The activities of glutathione S-transferases (GSTs) in serum and liver were tested under Vibrio anguillarum stress. The sigma-like glutathione S-transferases of Cyclina sinensis (CsGSTS) were cloned with SMART-cDNA library and large scale EST sequencing method, then the expression of CsGSTS in liver were detected by the real-time quantitative PCR. Results showed the GSTs activities in serum were up-regulated at 6—24h (P0.01) after infection, while in liver the activities declined at 6—12h and increased significantly at 48—96h. The full length cDNA of CsGSTS consisted of 793bp, encoding 206 amino acids with the typical GST-N and GST-C domain. The CsGSTS expression declined at 3—6h (P0.05), and increased significantly at 24h, then reached its highest level at 48h, which was about three times higher than control group. Results showed that Vibrio may cause tissue injury at the first stage of infection, then plenty of GSTs were produced and CsGSTS expressed intensively, both participating in the immune response of the clam. This investigation provided important experimental data for the study on immunologic mechanism in C. sinensis.