ECTO-enzyme activity of human erythrocyte adenosine deaminase

@article{Bielat2004ECTOenzymeAO,
  title={ECTO-enzyme activity of human erythrocyte adenosine deaminase},
  author={K L Bielat and George L. Tritsch},
  journal={Molecular and Cellular Biochemistry},
  year={2004},
  volume={86},
  pages={135-142}
}
SummaryAdenosine deaminase is found primarily in the cytoplasm of many cell types. In the human erythrocyte, about 30 per cent of the total adenosine deaminase activity is membrane associated, and about two-thirds of this is inactivated by treatment of intact erythrocytes with the nonpenetrating reagent diazotized sulfanilic acid, without affecting lactate dehydrogenase, a soluble cytoplasmic enzyme. This indicates that within the cell membranes, the catalytic site of about two-thirds of the… 
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References

SHOWING 1-10 OF 49 REFERENCES
Human adenosine deaminase. Distribution and properties.
[69] Specific adenosine deaminase from intestine
5'-Nucleotidase activity of mouse peritoneal macrophages. II. Cellular distribution and effects of endocytosis
TLDR
The diazonium salt of sulfanilic acid (DASA) can inactivate about 80% of the total 5'-nucleotidase of viable macrophages, which may reflect effects of the accumulated indigestible particles on the fate of incoming pinocytic vesicles or on newly synthesized plasma membrane precursor.
Studies on the specificity and mechanism of action of adenosine deaminase.
Partial purification and properties of the common inherited forms of adenosine deaminase from human erythrocytes.
TLDR
The partial purification of adenosine deaminase, types 1, 2 and 2-1, from human erythrocytes is described and heat inactivation of all forms of the enzyme was markedly dependent on ionic strength, the rate of inactivation increasing with increasing ionsic strength.
Immunoaffinity purification and fluorescence studies of human adenosine deaminase.
TLDR
While significant structural differences between adenosine deaminase from the two sources were evident, data indicate that both enzymes undergo conformational changes on binding ground-state and transition-state inhibitors.
Adenosine deaminase activity associated with phagocytic vacuoles
TLDR
Adenosine deaminase activity has been localized within the cell membrane and its surrounds phagocytic vacuoles in mouse macrophages, and is strategically located to direct metabolic flux through the enzymes of the purine catabolic pathway.
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