ECTO-enzyme activity of human erythrocyte adenosine deaminase

  title={ECTO-enzyme activity of human erythrocyte adenosine deaminase},
  author={K L Bielat and George L. Tritsch},
  journal={Molecular and Cellular Biochemistry},
SummaryAdenosine deaminase is found primarily in the cytoplasm of many cell types. In the human erythrocyte, about 30 per cent of the total adenosine deaminase activity is membrane associated, and about two-thirds of this is inactivated by treatment of intact erythrocytes with the nonpenetrating reagent diazotized sulfanilic acid, without affecting lactate dehydrogenase, a soluble cytoplasmic enzyme. This indicates that within the cell membranes, the catalytic site of about two-thirds of the… 

Enzymatic and extraenzymatic role of adenosine deaminase 1 in T-cell-dendritic cell contacts and in alterations of the immune function.

Recently studies performed with professional antigen-presenting cells and T lymphocytes have shown that ADA1 can bridge the two cell types together by a cross-linking established between different anchoring molecules in each cell.

Moonlighting Adenosine Deaminase: A Target Protein for Drug Development

ADA is considered as an example of this family of multifunctional proteins, and a mechanism by which some of their moonlighting functions can be coordinated is proposed, suggesting that drugs modulating ADA properties may act as modulators of theMoonlighting functions of ADA, giving them additional potential medical interest.

Adenosine transport and nitrobenzylthioinosine binding in human placental membrane vesicles from brush-border and basal sides of the trophoblast

It is concluded that the maternal (brush-border) and fetal (basal) surface of the human placental syncytiotrophoblast posses broad-specificity, facilitated-diffusion, NBMPR-sensitive nucleoside transporters.

Reversible binding of hemoglobin to band 3 constitutes the molecular switch that mediates O2 regulation of erythrocyte properties.

Comparison of transgenic mice with wild-type mice demonstrates that the following erythrocyte properties are controlled by the O2-dependent association of hemoglobin with band 3: assembly of a glycolytic enzyme complex on the ery throatyte membrane which is associated with a shift in glucose metabolism between the pentose phosphate pathway and Glycolysis, interaction of ankyrin with band3 and the concomitant regulation of ery Throcyte membrane stability.

Adenosine deaminase in the modulation of immune system and its potential as a novel target for treatment of inflammatory disorders.

Current data on the role played byADA in the regulation of immune system activity through its modulation of adenosine pathways is reviewed, with particular attention paid to the involvement of ADA in the pathophysiology of relevant inflammatory diseases.

Plasma Adenosine Deaminase (ADA)-1 and -2 Demonstrate Robust Ontogeny Across the First Four Months of Human Life

Overall, ADA isoforms demonstrate robust ontogeny in newborns and infants but further mechanistic studies are needed to clarify their roles in early life immune development and the correlations with sex, gestational age, and maternal age that were observed.


The aim of this paper is to present an overview of ADA deficiency and the available treatment modalities and to suggest antibiotic prophylaxis specific treatment provided based on individualized level.

Adenosine deaminase deficiency: current treatments and emerging therapeutics

GT should be considered for patients without an appropriate HSCT donor and when families are able to travel to Italy or participate in research protocols, although long-term follow up is needed to determine their continued effectiveness.



Human adenosine deaminase. Distribution and properties.

[69] Specific adenosine deaminase from intestine

5'-Nucleotidase activity of mouse peritoneal macrophages. II. Cellular distribution and effects of endocytosis

The diazonium salt of sulfanilic acid (DASA) can inactivate about 80% of the total 5'-nucleotidase of viable macrophages, which may reflect effects of the accumulated indigestible particles on the fate of incoming pinocytic vesicles or on newly synthesized plasma membrane precursor.

Studies on the specificity and mechanism of action of adenosine deaminase.

Partial purification and properties of the common inherited forms of adenosine deaminase from human erythrocytes.

The partial purification of adenosine deaminase, types 1, 2 and 2-1, from human erythrocytes is described and heat inactivation of all forms of the enzyme was markedly dependent on ionic strength, the rate of inactivation increasing with increasing ionsic strength.

Kinetics of the 5′-nucleotidase and the adenosine deaminase in subcellular fractions of rat brain

Kinetic parameters of the particulate 5′-nucleotidase and adenosine deaminase were analogous to those of the cytosolic enzymes, which suggest that soluble and particulate enzymes represent different pools of the same molecular species.

Immunoaffinity purification and fluorescence studies of human adenosine deaminase.

While significant structural differences between adenosine deaminase from the two sources were evident, data indicate that both enzymes undergo conformational changes on binding ground-state and transition-state inhibitors.