EB1 and APC bind to mDia to stabilize microtubules downstream of Rho and promote cell migration

  title={EB1 and APC bind to mDia to stabilize microtubules downstream of Rho and promote cell migration},
  author={Ying Wen and Christina H. Eng and Jan Schmoranzer and Noemí Cabrera-Poch and Edward J. Morris and M. Chen and Bradley J. Wallar and Arthur S. Alberts and Gregg G. Gundersen},
  journal={Nature Cell Biology},
Lysophosphatidic acid (LPA) stimulates Rho GTPase and its effector, the formin mDia, to capture and stabilize microtubules in fibroblasts. We investigated whether mammalian EB1 and adenomatous polyposis coli (APC) function downstream of Rho–mDia in microtubule stabilization. A carboxy-terminal APC-binding fragment of EB1 (EB1-C) functioned as a dominant-negative inhibitor of microtubule stabilization induced by LPA or active mDia. Knockdown of EB1 with small interfering RNAs also prevented… 

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