E. coli integration host factor binds to specific sites in DNA

  title={E. coli integration host factor binds to specific sites in DNA},
  author={N. Craig and H. Nash},
  • N. Craig, H. Nash
  • Published 1984
  • Biology, Medicine
  • Cell
  • E. coli integration host factor (IHF) both participates directly in phage lambda site-specific recombination and regulates the expression of phage and bacterial genes. Using protection from nuclease and chemical attack as an assay, we examined the interaction of IHF with DNA. We found that IHF is a specific DNA binding protein that interacts with three distinct segments of attP, the recombination site carried by phage lambda. We also found that specific IHF binding sites are located in non-att… CONTINUE READING
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