E. coli integration host factor binds to specific sites in DNA

@article{Craig1984ECI,
  title={E. coli integration host factor binds to specific sites in DNA},
  author={N. Craig and H. Nash},
  journal={Cell},
  year={1984},
  volume={39},
  pages={707-716}
}
  • N. Craig, H. Nash
  • Published 1984
  • Biology, Medicine
  • Cell
  • E. coli integration host factor (IHF) both participates directly in phage lambda site-specific recombination and regulates the expression of phage and bacterial genes. Using protection from nuclease and chemical attack as an assay, we examined the interaction of IHF with DNA. We found that IHF is a specific DNA binding protein that interacts with three distinct segments of attP, the recombination site carried by phage lambda. We also found that specific IHF binding sites are located in non-att… CONTINUE READING
    313 Citations

    Figures and Topics from this paper

    Integration host factor bends the DNA in the Escherichia coli ilvBN promoter region
    • 3
    Integration host factor stimulates the phage lambda pL promoter.
    • 64

    References

    SHOWING 1-10 OF 57 REFERENCES
    Purification and properties of the bacteriophage lambda Int protein.
    • H. Nash
    • Biology, Medicine
    • Methods in enzymology
    • 1983
    • 35
    Viral integration and excision: structure of the lambda att sites.
    • 194
    Purification and properties of Int-h, a variant protein involved in site-specific recombination of bacteriophage lambda.
    • 38
    Bacteriophage lambda int protein recognizes two classes of sequence in the phage att site: characterization of arm-type sites.
    • W. Ross, A. Landy
    • Biology, Medicine
    • Proceedings of the National Academy of Sciences of the United States of America
    • 1982
    • 83
    • PDF
    Purification of the bacteriophage lambda xis gene product required for lambda excisive recombination.
    • 76
    Am E. coli gene product required for λ site-specific recombination
    • 123
    • PDF
    Site-specific DNA condensation and pairing mediated by the int protein of bacteriophage lambda.
    • 79
    • PDF
    Multilevel regulation of bacteriophage λ lysogeny by the E. coli himA gene
    • 33