E. coli host strains significantly affect the quality of small scale plasmid DNA preparations used for sequencing.

@article{Taylor1993ECH,
  title={E. coli host strains significantly affect the quality of small scale plasmid DNA preparations used for sequencing.},
  author={Richard G. Taylor and David C Walker and Roderick R Mcinnes},
  journal={Nucleic acids research},
  year={1993},
  volume={21 7},
  pages={1677-8}
}
Several methods have been reported for the small scale preparation of plasmid DNA for sequencing (1-9), but little attention has been given to the effect of the E. coli host strain on the quality of the sequence obtained from double-stranded DNA. It has been observed that endonuclease A (endA) E.coli strains may produce nicked plasmid DNA resulting in 'shadow' bands on sequencing gels (1, 8), but no systematic evaluation of this phenomenon has been performed. We have found that of ten host… CONTINUE READING

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Ray.P.N., Mahuran.D.J. and McIimes.R.R

  • Taylor.R.G, Lambert.M.A, Sexsmith.E, S. J. Sadler
  • BioL Chem
  • 1990

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