E. coli SeqA protein binds oriC in two different methyl-modulated reactions appropriate to its roles in DNA replication initiation and origin sequestration

@article{Slater1995ECS,
  title={E. coli SeqA protein binds oriC in two different methyl-modulated reactions appropriate to its roles in DNA replication initiation and origin sequestration},
  author={S. Slater and S. Wold and M. Lu and E. Boye and K. Skarstad and N. Kleckner},
  journal={Cell},
  year={1995},
  volume={82},
  pages={927-936}
}
  • S. Slater, S. Wold, +3 authors N. Kleckner
  • Published 1995
  • Biology, Medicine
  • Cell
  • The seqA gene negatively modulates replication initiation at the E. coli origin, oriC. seqA is also essential for sequestration, which acts at oriC and the dnaA promoter to ensure that replication initiation occurs exactly once per chromosome per cell cycle. Initiation is promoted by full methylation of GATC sites clustered in oriC; sequestration is specific to the hemimethylated forms generated by replication. SeqA protein purification and DNA binding are described. SeqA interacts with fully… CONTINUE READING

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