E. coli K-12 asparaginase-based asparagine biosensor for leukemia.

  title={E. coli K-12 asparaginase-based asparagine biosensor for leukemia.},
  author={Neelam Singh Verma and K. Keerti Kumar and Gurnoor Kaur and Sneh Anand},
  journal={Artificial cells, blood substitutes, and immobilization biotechnology},
  volume={35 4},
The present work aims at the development of a novel, diagnostic biosensor for monitoring asparagine levels in leukemia. Various immobilization strategies have been applied to improve the stability of the biocomponent (asparaginase). Response time studies have been carried out for different immobilization methods. Phenol Red indicator has been coimmobilized with asparaginase and color visualization approach has been optimized for various asparagine ranges. The detection limit of asparagine… CONTINUE READING

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