E. coli DNA binding protein HU forms nucleosome-like structure with circular double-stranded DNA

  title={E. coli DNA binding protein HU forms nucleosome-like structure with circular double-stranded DNA},
  author={J. Rouvi{\`e}re-Yaniv and M. Yaniv and J. Germond},
  • J. Rouvière-Yaniv, M. Yaniv, J. Germond
  • Published 1979
  • Biology, Medicine
  • Cell
  • The incubation of the E coli DNA binding protein HU with relaxed circular SV40 DNA in the presence of pure nicking-closing enzyme introduces up to 18 negative superhelical turns in the DNA molecules as measured by agarose gel electrophoresis. The maximal density of supercoiling is obtained at a HU-DNA mass ratio of 1. Reconstituted DNA-HU complexes prefixed with glutaraldehyde appear as condensed circular structures having an average of 14 "beads" per circular SV40 DNA molecule, with a "bead… CONTINUE READING
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