E-probe Diagnostic Nucleic acid Analysis (EDNA): a theoretical approach for handling of next generation sequencing data for diagnostics.

@article{Stobbe2013EprobeDN,
  title={E-probe Diagnostic Nucleic acid Analysis (EDNA): a theoretical approach for handling of next generation sequencing data for diagnostics.},
  author={Anthony H. Stobbe and Jon Daniels and Andres Espindola and Ruchi Verma and U. Melcher and Francisco M. Ochoa-Corona and Carla Garzon and Jacqueline Fletcher and William Schneider},
  journal={Journal of microbiological methods},
  year={2013},
  volume={94 3},
  pages={356-66}
}
Plant biosecurity requires rapid identification of pathogenic organisms. While there are many pathogen-specific diagnostic assays, the ability to test for large numbers of pathogens simultaneously is lacking. Next generation sequencing (NGS) allows one to detect all organisms within a given sample, but has computational limitations during assembly and similarity searching of sequence data which extend the time needed to make a diagnostic decision. To minimize the amount of bioinformatic… CONTINUE READING
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