E. coli K-12 pel mutants, which block phage λ DNA injection, coincide with ptsM, which determines a component of a sugar transport system

Abstract

Escherichia coli pel - mutants inhibit the penetration of bacteriophage lambda DNA into the cell. Using P1 mediated cotransduction, we mapped pel - mutations between markers fadD and eda in the interval of minute 40 of the revised E. coli K-12 map. This places pel in the same region as genes kdgR and ptsM. Mutations in kdgR usually do not alter the Pel phenotype, and vice versa. In contrast, about 30% of ptsM - mutants are also pel -, and all pel - mutants isolated are ptsM -. These results suggest that pel and ptsM are one and the same gene. This interpretation would identify the bacterial product required for injection of phage λ DNA as a component of the phosphoenolpyruvate-dependent phosphotransferase system specific for mannose, glucosamine, glucose and fructose. However, the experimental results do not exclude an alternative explanation: that pel and ptsM identify two closely linked genes which would be simulataneously affected at high frequency by a particular mutational event.

DOI: 10.1007/BF00266608

4 Figures and Tables

Statistics

0100200'87'90'93'96'99'02'05'08'11'14'17
Citations per Year

540 Citations

Semantic Scholar estimates that this publication has 540 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Elliott1978ECK, title={E. coli K-12 pel mutants, which block phage λ DNA injection, coincide with ptsM, which determines a component of a sugar transport system}, author={Janet E. Elliott and Werner Arber}, journal={Molecular and General Genetics MGG}, year={1978}, volume={161}, pages={1-8} }