E‐Cadherin Transport from the trans‐Golgi Network in Tubulovesicular Carriers is Selectively Regulated by Golgin‐97

@article{Lock2005ECadherinTF,
  title={E‐Cadherin Transport from the trans‐Golgi Network in Tubulovesicular Carriers is Selectively Regulated by Golgin‐97},
  author={John G. Lock and Luke A. Hammond and Fiona J. Houghton and Paul A. Gleeson and Jennifer L. Stow},
  journal={Traffic},
  year={2005},
  volume={6}
}
E‐cadherin is a cell–cell adhesion protein that is trafficked and delivered to the basolateral cell surface. Membrane‐bound carriers for the post‐Golgi exocytosis of E‐cadherin have not been characterized. Green fluorescent protein (GFP)‐tagged E‐cadherin (Ecad‐GFP) is transported from the trans‐Golgi network (TGN) to the recycling endosome on its way to the cell surface in tubulovesicular carriers that resemble TGN tubules labeled by members of the golgin family of tethering proteins. Here, we… 
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121 Citations

The trans‐Golgi Network Golgin, GCC185, is Required for Endosome‐to‐Golgi Transport and Maintenance of Golgi Structure

A more effective long‐term depletion of GCC185 using miRNA than siRNA is demonstrated and a dual role for the GCC185 golgin in the regulation of endosome‐to‐TGN membrane transport and in the organization of the Golgi apparatus is demonstrated.

Golgin‐160 Promotes Cell Surface Expression of the Beta‐1 Adrenergic Receptor

The results support the idea that golgin‐160 may promote efficient surface delivery of a subset of cargo molecules and lead to significantly decreased cell surface levels of exogenously expressed β1‐adrenergic receptor (β1AR).

Amyloid precursor protein traffics from the Golgi directly to early endosomes in an Arl5b‐ and AP4‐dependent pathway

It is demonstrated that APP is diverted from BACE1 at the T GN for direct transport to early endosomes and that the TGN represents a site for APP processing with the subsequent secretion of Aβ.

ADP-ribosylation Factor-like GTPase ARFRP1 Is Required for Trans-Golgi to Plasma Membrane Trafficking of E-cadherin*

It is shown that in Arfrp1-/- embryos E-cadherin is mistargeted to intracellular compartments, whereas in control embryos it is present at the cell surface of trophectodermal and ectodermal cells, suggesting an essential role of the GTPase in trans-Golgi network function.

Golgin-97 Targets Ectopically Expressed Inward Rectifying Potassium Channel, Kir2.1, to the trans-Golgi Network in COS-7 Cells

Based on studies in COS-7 cells, it is proposed Golgi-97 facilitates formation of AP1-dependent export carriers for Kir2.1 by coupling anterograde delivery of Kir1 with retrograde recycling of AP-1 containing endosomes to the TGN.

A trans-Golgi network golgin is required for the regulated secretion of TNF in activated macrophages in vivo

These studies have identified p230 as a key regulator of TNF secretion and have shown that LPS activation of macrophages results in increased Golgi carriers for export, demonstrating a previously undescribed approach to control cytokine secretion by the specific silencing of trafficking machinery.

The golgin GCC88 is required for efficient retrograde transport of cargo from the early endosomes to the trans-Golgi network.

An essential role for GCC88 is identified in the localization of TGN fusion machinery for transport from early endosomes to the TGN, and the identification of a retrograde pathway which differentially selects TGN38 and mannose-6-phosphate receptor from Shiga toxin is allowed.

Regulation of apical vesicle formation from the trans-Golgi network

Comparing HA export to the release of basolateral cargo is compared, showing that nonpolarized cells are capable of differentially sorting distinct classes of cargo into discrete vesicles derived from the TGN, which further the understanding of the regulatory mechanisms underlying apical transmembrane protein export from theTGN.

Active Rab11 and functional recycling endosome are required for E-cadherin trafficking and lumen formation during epithelial morphogenesis.

During the development of epithelial cysts, DN-Rab11 disrupted E-cadherin targeting and trafficking, the subapical localization of pERM and actin, and cyst lumen formation, demonstrating an early and critical interdependence of Rab11 and the RE for E- cadher in targeting, apical membrane formation, and cell polarity in cysts.

Structure and domain organization of the trans- Golgi network

The trans-Golgi network is a unique compartment located at the exit face of the Golgi stack typically associated with a large amount of vesicular and tubular membranes and presents a trafficking hub where the secretory and endocytic pathways merge.
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