Dynein–Dynactin–NuMA clusters generate cortical spindle-pulling forces as a multi-arm ensemble

  title={Dynein–Dynactin–NuMA clusters generate cortical spindle-pulling forces as a multi-arm ensemble},
  author={M Okumura and Toyoaki Natsume and Masato T Kanemaki and Tomomi Kiyomitsu},
To position the mitotic spindle within the cell, dynamic plus ends of astral microtubules are pulled by membrane-associated cortical force-generating machinery. However, in contrast to the chromosome-bound kinetochore structure, how the diffusion-prone cortical machinery is organized to generate large spindle-pulling forces remains poorly understood. Here, we develop a light-induced reconstitution system in human cells. We find that induced cortical targeting of NuMA, but not dynein, is… CONTINUE READING


Publications citing this paper.


Publications referenced by this paper.
Showing 1-10 of 68 references

LGN/mInsc and LGN

J Zhu, W Wen, +7 authors M. Zhang
View 2 Excerpts
Highly Influenced

Molecular mechanism of dynein recruitment to kinetochores

AX, DK Cheerambathur, R. Gassmann

Similar Papers

Loading similar papers…