Dynein–Dynactin–NuMA clusters generate cortical spindle-pulling forces as a multi-arm ensemble

@inproceedings{Okumura2018DyneinDynactinNuMACG,
  title={Dynein–Dynactin–NuMA clusters generate cortical spindle-pulling forces as a multi-arm ensemble},
  author={M Okumura and Toyoaki Natsume and Masato T Kanemaki and Tomomi Kiyomitsu},
  booktitle={eLife},
  year={2018}
}
To position the mitotic spindle within the cell, dynamic plus ends of astral microtubules are pulled by membrane-associated cortical force-generating machinery. However, in contrast to the chromosome-bound kinetochore structure, how the diffusion-prone cortical machinery is organized to generate large spindle-pulling forces remains poorly understood. Here, we develop a light-induced reconstitution system in human cells. We find that induced cortical targeting of NuMA, but not dynein, is… CONTINUE READING

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