Dynamics of single mRNPs in nuclei of living cells.

  title={Dynamics of single mRNPs in nuclei of living cells.},
  author={Yaron Shav-Tal and Xavier Darzacq and Shailesh M. Shenoy and Dahlene Fusco and Susan M. Janicki and David L. Spector and Robert H Singer},
  volume={304 5678},
Understanding gene expression requires the ability to follow the fate of individual molecules. Here we use a cellular system for monitoring messenger RNA (mRNA)expression to characterize the movement in real time of single mRNA-protein complexes (mRNPs) in the nucleus of living mammalian cells. This mobility was not directed but was governed by simple diffusion. Some mRNPs were partially corralled throughout the nonhomogenous nuclear environment, but no accumulation at subnuclear domains was… CONTINUE READING
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