Dynamics of protozoa in the rumen of sheep.

  title={Dynamics of protozoa in the rumen of sheep.},
  author={R. A. Leng},
  journal={The British journal of nutrition},
  volume={48 2},
  • R. A. Leng
  • Published 1982 in The British journal of nutrition
Protozoa were labelled by incubating 100 ml rumen fluid with [14C]choline for 1 h. The protozoa were concentrated by centrifugation and then washed with rumen fluid. This reduced residual 14C in the fluid medium to insignificant amounts while still retaining the viability of the labelled protozoa. Washing procedures using formal saline (40 g formaldehyde/1 saline (9 g sodium chloride/1)) and saline were developed to isolate protozoa for estimation of specific radioactivity. 2. The protozoal… CONTINUE READING