Dynamics of paternal chromatin changes in live one-cell mouse embryo after natural fertilization.

@article{Adenot1991DynamicsOP,
  title={Dynamics of paternal chromatin changes in live one-cell mouse embryo after natural fertilization.},
  author={Pierre G. Adenot and Maria S. Sz{\"o}ll{\"o}si and Marc G{\`e}ze and J. P. Renard and Pascale Debey},
  journal={Molecular reproduction and development},
  year={1991},
  volume={28 1},
  pages={23-34}
}
Using video-enhanced fluorescence microscopy, we describe in live mouse zygotes the paternal chromatin changes undergone after fertilization. We focus on the sperm recondensation process and the formation of the paternal pronucleus, in relationship with the progression of maternal chromatin. Chromatin is labeled with the vital fluorophore Hoechst 33342. Our conditions of dye concentration and irradiation allow a continuous following of the dynamics of changes without major perturbation. We… CONTINUE READING

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