Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers.

@article{Kocher1989DynamicsOM,
  title={Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers.},
  author={Thomas D. Kocher and W. Kelley Thomas and Axel Meyer and Scott V. Edwards and Svante P{\"a}{\"a}bo and Francis X. Villablanca and A. C. C. Wilson},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1989},
  volume={86 16},
  pages={
          6196-200
        }
}
  • T. Kocher, W. Thomas, +4 authors A. Wilson
  • Published 1 August 1989
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
With a standard set of primers directed toward conserved regions, we have used the polymerase chain reaction to amplify homologous segments of mtDNA from more than 100 animal species, including mammals, birds, amphibians, fishes, and some invertebrates. [...] Key Result The bird and fish sequences evolve with the same strong bias toward transitions that holds for mammals. However, because the light strand of birds is deficient in thymine, thymine to cytosine transitions are less common than in other taxa. Amino…Expand
Versatile primers for the amplification of the mitochondrial DNA control region in marsupials
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