Dynamics of mitochondrial Ca2+ uptake in MICU1-knockdown cells.

  title={Dynamics of mitochondrial Ca2+ uptake in MICU1-knockdown cells.},
  author={Sergio de la Fuente and Jessica Matesanz-Isabel and Rosalba I. Fonteriz and Mayte Montero and Javier {\'A}lvarez},
  journal={The Biochemical journal},
  volume={458 1},
MICU1 (Ca2+ uptake protein 1, mitochondrial) is an important regulator of the MCU (Ca2+ uniporter protein, mitochondrial) that has been shown recently to act as a gatekeeper of the MCU at low [Ca2+]c (cytosolic [Ca2+]). In the present study we have investigated in detail the dynamics of MCU activity after shRNA-knockdown of MICU1 and we have found several new interesting properties. In MICU1-knockdown cells, the rate of mitochondrial Ca2+ uptake was largely increased at a low [Ca2+]c (<2 μM… CONTINUE READING