Dynamics of microglial activation: a confocal time-lapse analysis in hippocampal slices.

  title={Dynamics of microglial activation: a confocal time-lapse analysis in hippocampal slices.},
  author={Nick Stence and Marc Waite and Michael E. Dailey},
  volume={33 3},
The dynamics of microglial cell activation was studied in freshly prepared rat brain tissue slices. Microglia became activated in the tissue slices, as evidenced by their conversion from a ramified to amoeboid form within several hours in vitro. To define better the cytoarchitectural dynamics underlying microglial activation, we performed direct three-dimensional time-lapse confocal imaging of microglial cells in live brain slices. Microglia in tissue slices were stained with a fluorescent… CONTINUE READING
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