Dynamics of membrane protein/amphipol association studied by Förster resonance energy transfer: implications for in vitro studies of amphipol-stabilized membrane proteins.

@article{Zoonens2007DynamicsOM,
  title={Dynamics of membrane protein/amphipol association studied by F{\"o}rster resonance energy transfer: implications for in vitro studies of amphipol-stabilized membrane proteins.},
  author={Manuela Zoonens and Fabrice Giusti and Francesca Zito and J. L. Popot},
  journal={Biochemistry},
  year={2007},
  volume={46 36},
  pages={10392-404}
}
Amphipols (APols) are short amphipathic polymers that can substitute for detergents to keep membrane proteins (MPs) water-soluble while stabilizing them biochemically. We have examined the factors that determine the size and dispersity of MP/APol complexes and studied the dynamics of the association, taking as a model system the transmembrane domain of Escherichia coli outer membrane protein A (tOmpA) trapped by A8-35, a polyacrylate-based APol. Molecular sieving indicates that the solution… CONTINUE READING

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Membrane proteins from inclusion bodies to NMR spectra: the use of sulfonated amphipols (manuscript in preparation)

  • T. Dahmane, F. Giusti, L. J. Catoire, Popot, J.-L
  • 2007
Highly Influential
18 Excerpts

Ligand binding to membrane proteins immobilized onto solid supportsVia functionalized amphipols (manuscript in preparation)

  • D. Charvolin, F. Giusti, F. Rouvie r, Popot, J.-L
  • 2007
1 Excerpt

Protective and inhibitory effects of various types of amphipols on the Ca 2+ Dynamics of Membrane Protein/Amphipol

  • M. Picard, T. Dahmane, +6 authors P. Champeil
  • Association Biochemistry,
  • 2006

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