Dynamics of ligand-induced, Rac1-dependent anchoring of cadherins to the actin cytoskeleton

@article{Lambert2002DynamicsOL,
  title={Dynamics of ligand-induced, Rac1-dependent anchoring of cadherins to the actin cytoskeleton},
  author={Mireille Lambert and Daniel Choquet and Ren{\'e} Marc M{\`e}ge},
  journal={The Journal of Cell Biology},
  year={2002},
  volume={157},
  pages={469 - 479}
}
Cadherin receptors are key morphoregulatory molecules during development. To dissect their mode of action, we developed an approach based on the use of myogenic C2 cells and beads coated with an Ncad-Fc ligand, allowing us to mimic cadherin-mediated adhesion. We used optical tweezers and video microscopy to investigate the dynamics of N-cadherin anchoring within the very first seconds of bead-cell contact. The analysis of the bead movement by single-particle tracking indicated that N-cadherin… CONTINUE READING