Dynamics of induced CAT expression in E. coli.

  title={Dynamics of induced CAT expression in E. coli.},
  author={William E. Bentley and Robert H. Davis and Dhinakar S Kompala},
  journal={Biotechnology and bioengineering},
  volume={38 7},
The dynamics of chemically induced chloramphenicolaceyl-transferase (CAT) expression are determined in batch cultures of Escherichia coli DH5alphaF'IQ [pKK262-1]. This article is directed towards understanding the coupling of induced cloned-protein synthesis and reduced cell growth which are balanced in the optimal system. Experimental results indicate that the best inducer (IPTG) concentration is near 1.0 mM when added during midexponential growth. Lower concentrations cause only weak… CONTINUE READING


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