Dynamics of higher plant photosystem cross-section associated with state transitions

  title={Dynamics of higher plant photosystem cross-section associated with state transitions},
  author={Alexander V. Ruban and Matthew P. Johnson},
  journal={Photosynthesis Research},
Photosynthetic state transitions are a well-known phenomenon of short-term adaptation of the photosynthetic membrane to changes in spectral quality of light in low light environments. The principles of the monitoring and quantification of the process in higher plants are revised here. The use of the low-temperature excitation fluorescence spectroscopy for analysis of the photosystem I antenna cross-section dynamics is described. This cross section was found to increase by 20–25% exclusively due… 

Live-cell imaging of photosystem II antenna dissociation during state transitions

Induction of energy redistribution in wild-type cells led to an increase in, and spreading of, a 250-ps lifetime chlorophyll fluorescence component, which was not observed in the stt7 mutant incapable of state transitions, and was also the dominant component in a mutant containing the light-harvesting antenna complexes but no photosystems.

Probing functional and optical cross-sections of PSII in leaves during state transitions using fast repetition rate light induced fluorescence transients.

The extent and dynamics of ST in leaves are monitored from estimates of optical absorption cross-section (relative PSII antenna size; aPSII) and maximum photochemical efficiency of PSII (φPSII); i.e, the extent of ST is deduced from changes in a'PSII and other parameters with reduced QA range up to 35%.

Light-harvesting processes in the dynamic photosynthetic antenna.

From the theoretical basis of light-harvesting within the photosynthetic membrane, fluorescence recovery after photo-bleaching as a technique for visualising membrane mobility is discussed, before introducing light-induced grana membrane reorganisation as an integral part of the rapid photoprotective switch in plants.

Absence of photosynthetic state transitions in alien chloroplasts

A mechanistic explanation is provided for the lack of state transitions in a Nt(Hn) cybrid—the N-terminal truncation of the Lhcb proteins in the cybrid included the threonine residue that is phosphorylated/dephosphorylated in order to trigger state transitions and therefore crucial energy balancing mechanism in plants.

Changes in antenna sizes of photosystems during state transitions in granal and stroma-exposed thylakoid membrane of intact chloroplasts in Arabidopsis mesophyll protoplasts.

Subdomain analysis of granal and stromal thylakoid regions clarified variant behaviors in the different regions of light-harvesting complex II and determined amplitude changes of PSI and PSII in fluorescence spectra solely due to state transition.

State transitions--the molecular remodeling of photosynthetic supercomplexes that controls energy flow in the chloroplast.

  • J. Minagawa
  • Environmental Science
    Biochimica et biophysica acta
  • 2011

Dynamic thylakoid stacking regulates the balance between linear and cyclic photosynthetic electron transfer

It is shown that changes in thylakoid stacking regulate the balance between LET and CET pathways, which alters thylakhoid membrane architecture and the organization of photosynthetic complexes, without significantly affecting the antenna size of either photosystem.

Steady-State Phosphorylation of Light-Harvesting Complex II Proteins Preserves Photosystem I under Fluctuating White Light1[W][OA]

It is demonstrated that the Arabidopsis (Arabidopsis thaliana) stn7 (for state transition7) mutant, devoid of the STN7 kinase and LHCII phosphorylation, shows a growth penalty only under fluctuating white light due to a low amount of PSI.



Configuration and Dynamics of Xanthophylls in Light-harvesting Antennae of Higher Plants

Both xanthophylls (notably, zeaxanthin) were found to be well coordinated within the antenna proteins in vivo, arguing against the possibility of their free diffusion in the membrane and supporting the recent biochemical evidence of their association with intact oligomeric light-harvesting complexes.

Structural characterization of a complex of photosystem I and light-harvesting complex II of Arabidopsis thaliana.

This work studied the structures and frequencies of all major membrane complexes from Arabidopsis thaliana chloroplasts at conditions favoring either state 1 or state 2, showing that LHCII has a specific binding site at the PSI-A, -H, -L, and -K subunits.

Phosphorylation-dependent regulation of excitation energy distribution between the two photosystems in higher plants.

Heat-induced reversible changes in Photosystem 1 absorption cross-section of pea chloroplasts and sub-chloroplast preparations

Reversible changes in the room temperature fluorescence quenching at 685 nm and light scattering level at 577 nm, indicating about 15% of granal unstacking, induced by high temperature treatment of pea chloroplasts were shown, may indicate the heterogeneous character of the temperature-induced energy redistribution across the PS 1-containing chloroplast membrane compartments.