Dynamics of fluorescence marker concentration as a probe of mobility.

@article{Koppel1976DynamicsOF,
  title={Dynamics of fluorescence marker concentration as a probe of mobility.},
  author={Dennis E. Koppel and Daniel Axelrod and Joseph Schlessinger and Elliot L. Elson and Watt W. Webb},
  journal={Biophysical journal},
  year={1976},
  volume={16 11},
  pages={1315-29}
}
We have developed an effective experimental system for the characterization of molecular and structural mobility. It incorporates a modified fluorescence microscope geometry and a variety of analytical techniques to measure effective diffusion coefficients ranging over almost six orders of magnitude, from less than 10(-11) cm2/s to greater than 10(-6) cm2/s. Two principal techniques, fluorescence correlation spectroscopy (FCS) and fluorescence photobleaching recovery (FPR), are employed. In the… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 86 extracted citations

Fluorescence correlation spectroscopy for the detection and study of single molecules in biology.

BioEssays : news and reviews in molecular, cellular and developmental biology • 2002
View 2 Excerpts
Highly Influenced

Methods in Membrane Lipids

Methods in Molecular Biology • 2015

Similar Papers

Loading similar papers…