Dynamics of fluorescence marker concentration as a probe of mobility.

  title={Dynamics of fluorescence marker concentration as a probe of mobility.},
  author={Dennis E. Koppel and Daniel Axelrod and Joseph Schlessinger and Elliot L. Elson and Watt W. Webb},
  journal={Biophysical journal},
  volume={16 11},
We have developed an effective experimental system for the characterization of molecular and structural mobility. It incorporates a modified fluorescence microscope geometry and a variety of analytical techniques to measure effective diffusion coefficients ranging over almost six orders of magnitude, from less than 10(-11) cm2/s to greater than 10(-6) cm2/s. Two principal techniques, fluorescence correlation spectroscopy (FCS) and fluorescence photobleaching recovery (FPR), are employed. In the… CONTINUE READING


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