Dynamics of a fluorescent calmodulin analog in the mammalian mitotic spindle at metaphase.

@article{Stemple1988DynamicsOA,
  title={Dynamics of a fluorescent calmodulin analog in the mammalian mitotic spindle at metaphase.},
  author={Derek L. Stemple and S C Sweet and Michael J. Welsh and J Richard McIntosh},
  journal={Cell motility and the cytoskeleton},
  year={1988},
  volume={9 3},
  pages={231-42}
}
We have compared the exchange kinetics of fluorescein-labeled calmodulin and tubulin in the spindles of living mitotic cells at metaphase. Cultured mammalian cells in early stages of mitosis were microinjected with labeled calmodulin or tubulin and returned to an incubator to allow equilibration of the fluorescent protein with the endogenous protein pools. Calmodulin becomes concentrated in the mitotic spindle, and treatments with inhibitors of tubulin assembly show that this concentration is… CONTINUE READING

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