Dynamics of Translation of Single mRNA Molecules In Vivo

  title={Dynamics of Translation of Single mRNA Molecules In Vivo},
  author={Xiaowei Yan and Tim A. Hoek and Ronald D. Vale and Marvin E. Tanenbaum},
Regulation of mRNA translation, the process by which ribosomes decode mRNAs into polypeptides, is used to tune cellular protein levels. Currently, methods for observing the complete process of translation from single mRNAs in vivo are unavailable. Here, we report the long-term (>1 hr) imaging of single mRNAs undergoing hundreds of rounds of translation in live cells, enabling quantitative measurements of ribosome initiation, elongation, and stalling. This approach reveals a surprising… CONTINUE READING
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