Dynamics of O2 consumption in rat pancreatic islets

The O2 consumption of rat pancreatic islets was determined by monitoring pO2 in the perifusate from groups of 200–300 islets. Basal respiration was maintained for up to 2 h. The insulin secretagogues, glucose and 4-methyl-2-oxopentanoate, provoked an immediate (<5 s) increase in islet respiration which attained a new steady-state within 10–40 min. The… CONTINUE READING