Dynamics of L-929 cell spreading after mitosis

Abstract

Changes in the cell shape of L-929 (NCTC, clone 929) during the cell cycle were analyzed with time-lapse microscopy. It was found that the cells pass through three spreading stages. The maximal cell spreading was observed during the first 1.5 h after mitosis. In this period, the cell area increases in correspondence with the sigmoid dependence and enlarges by approximately 3.0–3.5 times. After a short plateau, the cell area begins to increase, also correspondence with the sigmoid dependence. This period is longer (up to 6 h after the beginning of cell division), with an additional 1.5-fold increase in the cell size. Later, cell area enlargement continues linearly up to the beginning of the next mitosis. After the mother L-929 cell division, almost 100% of daughter cells remained to be bridged together in the site of the division furrow. Connected L-929 cells spread and migrate for 2.13 ± 0.06 h and then separate. The daughter-cell shape transition from round to the spread one and simultaneous maintenance of the intercellular bridge during a strictly determined time allows us to consider this phenomenon as independent and not related to mitosis. We suggest designating this junction between the daughter cells as the “posttelophase intercellular bridge.”

DOI: 10.1134/S1990519X12040086

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@article{Petrov2012DynamicsOL, title={Dynamics of L-929 cell spreading after mitosis}, author={Yu. P. Petrov and Yu. A. Negulyaev and Natalia Tsupkina}, journal={Cell and Tissue Biology}, year={2012}, volume={6}, pages={383-388} }