Dynamics of γH2AX formation and elimination in mammalian cells after X-irradiation.

@article{Firsanov2012DynamicsO,
  title={Dynamics of γH2AX formation and elimination in mammalian cells after X-irradiation.},
  author={Denis V. Firsanov and Anastasia A. Vasilishina and Andrey V Kropotov and Vyacheslav M. Mikhailov},
  journal={Biochimie},
  year={2012},
  volume={94 11},
  pages={2416-22}
}
Phosphorylation of the replacement histone H2AX occurs in megabase chromatin domains around DNA double-strand breaks (DSBs), and this modification called γH2AX can be used as an effective marker for DSB repair and DNA damage response. In this study, we examined a bystander effect (BE) in locally irradiated embryonic human fibroblasts. Using fluorescence microscopy, we found that BE could be observed 1 h after X-ray irradiation (IR) and was completely eliminated 24 h after IR. Using… CONTINUE READING