Dynamic properties of the active site of azurin studied by the temperature dependence of the optical spectrum


We report the optical absorption spectra of azurin (Pseudomonas aeruginosa) in the temperature range 290-20 K. The samples used are protein aqueous solutions containing 65% (by Vol.) glycerol as cryoprotectant. The measured spectra are deconvoluted in gaussian components and the temperature dependence of the zeroth, first and second moment of the observed… (More)
DOI: 10.1007/BF01179507


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