Dynamic nuclear polarization-enhanced solid-state NMR of a 13C-labeled signal peptide bound to lipid-reconstituted Sec translocon.

@article{Reggie2011DynamicNP,
  title={Dynamic nuclear polarization-enhanced solid-state NMR of a 13C-labeled signal peptide bound to lipid-reconstituted Sec translocon.},
  author={Lenica Reggie and Jakob J. Lopez and Ian Collinson and Clemens Glaubitz and Mark Lorch},
  journal={Journal of the American Chemical Society},
  year={2011},
  volume={133 47},
  pages={19084-6}
}
Dynamic nuclear polarization (DNP) has made it possible to record 2D double-quantum-filtered (DQF) solid-state NMR (ssNMR) spectra of a signal peptide bound to a lipid-reconstituted SecYEG translocon complex. The small quantity of peptide in the sample (~40 nmol) normally prohibits multidimensional ssNMR experiments. Such small amounts are not the exception, because for samples involving membrane proteins, most of the limited sample space is occupied by lipids. As a consequence, a conventional… CONTINUE READING
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