Dynamic instability of individual microtubules analyzed by video light microscopy: rate constants and transition frequencies

@article{Walker1988DynamicIO,
  title={Dynamic instability of individual microtubules analyzed by video light microscopy: rate constants and transition frequencies},
  author={Richard A. Walker and Edward T O'Brien and N K Pryer and M F Soboeiro and William A. Voter and Harold P Erickson and Edward D. Salmon},
  journal={The Journal of Cell Biology},
  year={1988},
  volume={107},
  pages={1437 - 1448}
}
We have developed video microscopy methods to visualize the assembly and disassembly of individual microtubules at 33-ms intervals. Porcine brain tubulin, free of microtubule-associated proteins, was assembled onto axoneme fragments at 37 degrees C, and the dynamic behavior of the plus and minus ends of microtubules was analyzed for tubulin concentrations between 7 and 15.5 microM. Elongation and rapid shortening were distinctly different phases. At each end, the elongation phase was… CONTINUE READING
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