Dynamic expression of de novo DNA methyltransferases Dnmt3a and Dnmt3b in the central nervous system

@article{Feng2005DynamicEO,
  title={Dynamic expression of de novo DNA methyltransferases Dnmt3a and Dnmt3b in the central nervous system},
  author={Jian Feng and Hua Chang and En Li and Guoping Fan},
  journal={Journal of Neuroscience Research},
  year={2005},
  volume={79}
}
To explore the role of DNA methylation in the brain, we examined the expression pattern of de novo DNA methyltransferases Dnmt3a and Dnmt3b in the mouse central nervous system (CNS. [] Key Method To determine the precise pattern of Dnmt3a and Dnmt3b gene expression, we carried out X-gal histochemistry in transgenic mice in which the lacZ marker gene is knocked into the endogenous Dnmt3a or Dnmt3b gene locus (Okano et al. [1999] Cell 99:247-257). In Dnmt3b-lacZ transgenic mice, X-gal-positive cells are…
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The results indicate that DNMT3B mediates large-scale methylation patterns in hESCs and that DN MT3B deficiency in the cells alters the timing of their neuronal differentiation and maturation.
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Dnmt 1 and Dnmt 3 a are required for the maintenance of DNA methylation and synaptic function in adult forebrain neurons
TLDR
It is concluded that DnMT1 and Dnmt3a are required for synaptic plasticity, learning and memory through their overlapping roles in maintaining DNA methylation and modulating neuronal gene expression in adult CNS neurons.
A DNMT3A PWWP mutation leads to methylation of bivalent chromatin and growth retardation in mice
TLDR
A mouse model carrying a D329A point mutation in the DNMT3A PWWP domain is presented and it is found this causes dominant postnatal growth retardation, with aberrant progressive gain of DNA methylation across domains marked by H3K27me3 in adult tissues.
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References

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