Dynamic characterization of the water binding loop in the P-type cardiotoxin: implication for the role of the bound water molecule.

@article{Sue2001DynamicCO,
  title={Dynamic characterization of the water binding loop in the P-type cardiotoxin: implication for the role of the bound water molecule.},
  author={S C Sue and Harold C. Jarrell and J. R. Brisson and Wan-Shian Wu},
  journal={Biochemistry},
  year={2001},
  volume={40 43},
  pages={12782-94}
}
Recent studies of cobra P-type cardiotoxins (CTXs) have shown that the water-binding loop (loop II) plays a crucial role in toxin binding to biological membranes and in their cytotoxicity. To understand the role of bound water in the loop, the structure and dynamics of the major P-type CTX from Taiwan cobra, CTX A3, were determined by a comprehensive NMR analysis involving (1)H NOESY/ROESY, (13)C[1)H]NOE/T(1) relaxation, and (17)O triple-quantum filtered NMR. A single water molecule was found… CONTINUE READING