Dynamic behavior of transcription factors on a natural promoter in living cells.

@article{Becker2002DynamicBO,
  title={Dynamic behavior of transcription factors on a natural promoter in living cells.},
  author={Matthias Becker and Christopher T. Baumann and S Rose John and Dawn A Walker and Marc Vigneron and James G. McNally and Gordon L. Hager},
  journal={EMBO reports},
  year={2002},
  volume={3 12},
  pages={1188-94}
}
Through the use of photobleaching techniques, we examined the dynamic interaction of three members of the transcription apparatus with a target promoter in living cells. The glucocorticoid receptor (GR) interacting protein 1 (GRIP-1) exhibits a half maximal time for fluorescent recovery (tau(R)) of 5 s, reflecting the same rapid exchange as observed for GR. In contrast, the large subunit (RPB1) of RNA polymerase II (pol II) required 13 min for complete fluorescence recovery, consistent with its… CONTINUE READING