Dynamic assembly of end-joining complexes requires interaction between Ku70/80 and XRCC4.

  title={Dynamic assembly of end-joining complexes requires interaction between Ku70/80 and XRCC4.},
  author={Pierre-Olivier Mari and Bogdan I. Florea and Stephan P. Persengiev and Nicole S. Verkaik and Hennie T. Br{\"u}ggenwirth and Mauro Modesti and Giuseppina Giglia-Mari and Karel Bezstarosti and Jeroen A A Demmers and Theo M. Luider and Adriaan B. Houtsmuller and Dik C van Gent},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={103 49},
DNA double-strand break (DSB) repair by nonhomologous end joining (NHEJ) requires the assembly of several proteins on DNA ends. Although biochemical studies have elucidated several aspects of the NHEJ reaction mechanism, much less is known about NHEJ in living cells, mainly because of the inability to visualize NHEJ repair proteins at DNA damage. Here we provide evidence that a pulsed near IR laser can produce DSBs without any visible alterations in the nucleus, and we show that NHEJ proteins… CONTINUE READING
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XRCC4 protein, humanGene product plays role in biological processNon-Homologous DNA End-Joining
Our results suggest that this assembly constitutes the core of the NHEJ reaction and that XRCC4 may serve as a flexible tether between Ku70/80 and ligase IV .
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